In the Process of Isolating DNA for Transcription and Translation,you

question 24

Multiple Choice

In the process of isolating DNA for transcription and translation,you digest the DNA with BamHI and ligate the gene into a plasmid vector.However,even though all of the controls work,no transcription is obtained using your fragment.Working backward,you note that restriction digestion has removed approximately 250 base pairs from the 5' end of the gene.It is possible that during restriction digestion

• A)the termination signal was removed.
• B)the RNA polymerase became inactivated.
• C)primase could not bind.
• D)the promoter was removed.
• E)none of the above

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Definitions:

Accrual Accounting

An accounting method that records revenues and expenses when they are incurred, regardless of when cash transactions occur, providing a more accurate picture of a company's financial health.

Diversification

A risk management strategy that mixes a wide variety of investments within a portfolio or a company's product line to minimize risks.

Alternative Accounting Policies

Different methods that an organization can use for reporting similar transactions or economic events in financial statements, based on accepted accounting principles.

Discontinued Operations

Components of a business that have been sold or otherwise disposed of, or will be disposing of, which are reported separately in financial statements.