What Is the Purpose of Treating a Protein with 2-Mercaptoethanol

question 34

Multiple Choice

What is the purpose of treating a protein with 2-mercaptoethanol?

• A)To hydrolyze the protein into its amino acids.
• B)To cleave the disulfide bonds.
• C)To derivatize any free sulfhydryl groups to prevent them from reforming disulfide bonds.
• D)To derivatize the N-terminal amino acid during the Edman degradation.

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