Which Might Be Most Useful for Determining the Amino Acid

question 51

Multiple Choice

Which might be most useful for determining the amino acid residues in an enzyme's active site?

• A)Use an irreversible inhibitor that reacts with the side chains of specific amino acids.
• B)Determine the entire primary structure of the protein.
• C)Use a competitive inhibitor and plot the rate vs concentration of inhibitor.
• D)Unfold the enzyme with urea and 2-mercaptoethanol.Then incubate and crystallize it in the presence of the substrate followed by X-ray crystallography.