What Is the Most Logical Sequence of Steps for Splicing

question 27

Multiple Choice

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes.
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V. Use ligase to seal plasmid DNA to nonplasmid DNA.

• A)I, II, IV, III, V
• B)II, III, V, IV, I
• C)III, II, IV, V, I
• D)III, IV, V, I, II
• E)IV, V, I, II, III

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Definitions:

Primary Data

Primary data refers to original and direct data collected by researchers through observations, surveys, experiments, or other methods specifically for their study.

Open-ended Questions

Questions framed in a way that requires a detailed, descriptive answer, allowing for the expression of thoughts, feelings, or opinions, beyond a simple yes or no.

Qualitatively

Pertaining to or concerned with descriptions and distinctions made in terms of qualities rather than quantitative measurements.

Hypothesis Testing

A method used in scientific research to determine if there is sufficient evidence to support a predicted relationship or effect.