Use the Following Information to Answer the Questions Below

question 50

Multiple Choice

Use the following information to answer the questions below.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
-Bacteria that do not take up any plasmids would grow on which media?

• A)the nutrient broth only
• B)the nutrient broth and the tetracycline broth
• C)the nutrient broth and the ampicillin broth
• D)the tetracycline broth and the ampicillin broth
• E)all four broths

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Definitions:

Common Stockholders

Owners of common stock in a corporation, who have voting rights and may receive dividends, but are typically last in line to receive assets in the event of liquidation.

Stockholders' Equity

The residual assets of a company that belong to the shareholders, calculated as total assets minus total liabilities.

Liabilities

Financial obligations or debts that a company owes to others, reflecting amounts that must be paid in the future.

Authorized Shares

The maximum number of shares a corporation is legally allowed to issue, as stipulated in its articles of incorporation.