Use the Following Information to Answer the Questions Below

question 65

Multiple Choice

Use the following information to answer the questions below.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
-A principal problem with inserting an unmodified mammalian gene into a bacterial plasmid, and then getting that gene expressed in bacteria, is that

• A)prokaryotes use a different genetic code from that of eukaryotes.
• B)bacteria translate polycistronic messages only.
• C)bacteria cannot remove eukaryotic introns.
• D)bacterial RNA polymerase cannot make RNA complementary to mammalian DNA.
• E)bacterial DNA is not found in a membrane-bounded nucleus and is therefore incompatible with mammalian DNA.