What Is the Most Logical Sequence of Steps for Splicing

question 27

Multiple Choice

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes.
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V. Use ligase to seal plasmid DNA to nonplasmid DNA.

• A)I, II, IV, III, V
• B)II, III, V, IV, I
• C)III, II, IV, V, I
• D)III, IV, V, I, II
• E)IV, V, I, II, III

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Definitions:

Preemption

A legal doctrine under which a higher authority's laws take precedence over those of a lower authority when both entities have the power to legislate on a subject.

Mootness

A concept whereby a case is deemed inadmissible for judicial review if the underlying issue has been resolved or rendered irrelevant.

Ripeness

A criterion used in constitutional law to determine if a court case is premature for review because the issues involved have not yet fully developed.

Impossibility

A defense in contract law where a party claims that fulfilling the contractual obligations has become objectively impossible due to unforeseen events.