Use the Following Information to Answer the Questions Below

question 50

Multiple Choice

Use the following information to answer the questions below.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
-Bacteria that do not take up any plasmids would grow on which media?

• A)the nutrient broth only
• B)the nutrient broth and the tetracycline broth
• C)the nutrient broth and the ampicillin broth
• D)the tetracycline broth and the ampicillin broth
• E)all four broths

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Definitions:

Unique Identity

Unique identity refers to the distinctive characteristics or attributes that make an individual, group, or entity different from others.

Unique Identity

A distinct characteristic or combination of characteristics that distinguishes an individual, group, or entity from others.

Institutional Adaptation

Institutional adaptation refers to the adjustments that institutions make to cope with external shifts or internal developments, aiming to maintain stability and continue serving their functions effectively.

Multibased Socialization

The process through which individuals learn and adopt the values, norms, and required behaviors of multiple groups within an organization.