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Use the Following Information to Answer the Questions Below

question 65

Multiple Choice

Use the following information to answer the questions below.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
-A principal problem with inserting an unmodified mammalian gene into a bacterial plasmid, and then getting that gene expressed in bacteria, is that

Definitions:

Hydrophilic

Describes molecules or substances that are attracted to water and dissolve well in it.

Linoleic Acid

An essential polyunsaturated fatty acid belonging to the omega-6 fatty acid family, necessary for human health but cannot be synthesized by the human body.

HBr

Hydrobromic acid, a strong acid used in organic synthesis, notably for the addition of bromine across double bonds in alkenes.

KMnO4

Potassium permanganate, a strong oxidizing agent used in various chemical reactions, including organic synthesis and analytical chemistry.