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Can P Bursaria Live in Association with Any and All Strains/species

question 63

Multiple Choice

Can P. bursaria live in association with any and all strains/species of Chlorella? In an experiment to help answer this question, Chlorella was collected, and cultured separately, from three different sources: (1) P. bursaria cytoplasm, (2) free-living Chlorella, and (3) from cytoplasm of other protist species. A population of P. bursaria was treated with the herbicide, paraquat, which killed all of its zoochlorellae, but otherwise left P. bursaria unharmed. The zoochlorella-free paramecia were then introduced to a 1:1:1 mixture of Chlorella from the three cultures listed above, and subsequently reestablished a contingent of zooclorellae. Two weeks later, zoochlorellae were collected from the P. bursaria cells and tested to determine which Chlorella strain(s) had been maintained within P. bursaria. The different strains of Chlorella are morphologically indistinguishable. Consequently, which of these would be the best test to perform on Chlorella, both before and after re-establishment of zoochlorellae, to determine which Chlorella strains had been maintained within P. bursaria?

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