A Geneticist Introduces a Transgene into Yeast Cells and Isolates

question 28

Multiple Choice

A geneticist introduces a transgene into yeast cells and isolates five independent cell lines in which the transgene has integrated into the yeast genome. In four of the lines, the transgene is expressed strongly, but in the fifth there is no expression at all.
-Which of the following is a likely explanation for the lack of transgene expression in the fifth cell line?

• A) A transgene integrated into a heterochromatic region of the genome.
• B) A transgene integrated into a euchromatic region of the genome.
• C) The transgene was mutated during the process of integration into the host cell genome.
• D) The host cell lacks the enzymes necessary to express the transgene.
• E) A transgene integrated into a region of the genome characterized by high histone acetylation.

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Definitions:

Bed-wetting

Involuntary urination during sleep, commonly seen in children.

Classical Conditioning

The mechanism of learning through the linkage of a natural stimulus and an environmental one.

Cravings

Intense desires or urges for a specific substance, experience, or activity, often associated with addiction or deprivation.

Counterconditioning

A behavior therapy technique involving the conditioning of an unwanted behavior or response to a stimulus into a desired behavior or response.