What Is the Most Logical Sequence of Steps for Splicing

question 8

Multiple Choice

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.Transform bacteria with a recombinant DNA molecule.
II.Cut the plasmid DNA using restriction enzymes.
III.Extract plasmid DNA from bacterial cells.
IV.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V.Use ligase to seal plasmid DNA to nonplasmid DNA.

• A) I, II, IV, III, V
• B) II, III, V, IV, I
• C) III, II, IV, V, I
• D) III, IV, V, I, II
• E) IV, V, I, II, III

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Definitions:

Direct Costs

Expenses that can be directly traced to a product or service, such as materials and labor.

Inventory Costing Methods

Refers to the techniques used to determine the value of an inventory, including LIFO (Last In, First Out), FIFO (First In, First Out), and weighted average methods.

Consigned Merchandise

Goods that are sent by one party to another to sell, with payment to the consignor occurring only after the sale.

Consignee's Inventory

Goods that are held by a consignee but still legally belong to the consignor until sold, after which sales revenue is shared as per agreement.