A Principal Problem with Inserting an Unmodified Mammalian Gene into a Plasmid

question 46

Multiple Choice

A principal problem with inserting an unmodified mammalian gene into a plasmid, and then getting that gene expressed in bacteria, is that

• A)prokaryotes use a different genetic code from that of eukaryotes.
• B)bacteria translate polycistronic messages only.
• C)bacteria cannot remove eukaryotic introns.
• D)bacterial RNA polymerase cannot make RNA complementary to mammalian DNA.
• E)bacterial DNA is not found in a membrane-bounded nucleus and is therefore incompatible with mammalian DNA.

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Definitions:

Peripherally Inserted Central Catheter (PICC)

A long, thin tube inserted through a vein in the arm, extending to the central veins near the heart, used for prolonged intravenous access.

Implanted Port Catheter

A medical device surgically inserted under the skin, used to administer medication or fluids, or to draw blood, providing easy access to veins.

Sepsis

A life-threatening medical condition caused by an overwhelming immune response to infection, leading to tissue damage, organ failure, and potentially death.

Continuous Infusion

The delivery of medication or fluids into the bloodstream at a constant rate over an extended period of time.