Which Procedure Is Not Required When the Shotgun Approach to Sequencing

question 13

Multiple Choice

Which procedure is not required when the shotgun approach to sequencing is modified as sequencing by synthesis, in which many small fragments are sequenced simultaneously?

• A) use of restriction enzymes
• B) sequencing each fragment
• C) cloning each fragment into a plasmid
• D) ordering the sequences
• E) PCR amplification

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Definitions:

Total Output

The total value of all goods and services produced in an economy over a specific time period.

Average Total Cost

The total cost of production divided by the quantity produced, incorporating both fixed and variable costs.

Average Fixed Cost

The constant costs of producing (which remain the same regardless of output volume) shared among the produced output units.

Average Variable Cost

Variable costs, which fluctuate based on production volume, divided by the total number of units produced.