A Technique Called Flow Cytometry Is Used by Scientists and Researchers

question 35

Multiple Choice

A technique called flow cytometry is used by scientists and researchers to count or sort cells based on specific properties. By labeling cellular DNA with a fluorescent dye, flow cytometry can sort cells based on the amount of DNA present, thereby making it possible to distinguish between cells that are in different stages of the cell cycle. Flow cytometry is especially useful to the medical community because it can help with the diagnosis of certain types of cancers. Below is a typical set of data that you might obtain when running a flow cytometry experiment using a sample of healthy skin cells. Use this figure to help you answer the following questions. After reading the paragraph below, answer the questions that follow.
-If you used flow cytometry to sort a sample of cancerous cells that have been treated with a drug to prevent them from replicating their DNA, what peaks would you expect to see on the resulting flow cytometry data set?

• A) A only
• B) C only
• C) A and B only
• D) A, B, and C

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Definitions:

Melancholic

A temperament characterized by deep, pensive sadness or contemplation, often connected to artistic or creative endeavors.

Phlegmatic

A temperament characterized by a calm, reliable, and even-keeled personality, often associated with a high threshold for emotional excitability.

Sanguine

Characterized by optimism or a positive attitude, especially in an apparently bad or difficult situation.

Self-Efficacy

An individual's belief in their capacity to execute behaviors necessary to produce specific performance attainments.