After Reading the Paragraph Below, Answer the Questions That Follow

question 62

Multiple Choice

After reading the paragraph below, answer the questions that follow.
While DNA microarrays can be used to determine whether or not a gene is expressed in a cell, quantitative polymerase chain reaction (qPCR) can be used to measure the extent of gene expression in cells and tissues. To perform qPCR, mRNA is extracted from cells and reverse transcribed to complementary DNA (cDNA) . Using fluorescent DNA molecules that recognize specific gene sequences and DNA polymerase, copies of the gene sequence of interest are made. After the copies are made, a fluorescent detector measures the intensity of the fluorescent signal, which is proportional to the number of mRNA transcripts in the cell and corresponds to the level of gene expression in the cell.
-Which of the following statements regarding the comparison of DNA microarrays and qPCR is false?

• A)Both methods use fluorescent DNA molecules and cDNA.
• B)Both methods can determine whether a gene has been turned on or not.
• C)Both methods use mRNA extracted from cells.
• D)Both methods can quantify the levels of gene expression in cells.

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Definitions:

Aqueous Produced

Refers to the production of aqueous humor, a clear fluid in the eye that maintains eye pressure and provides nutrients to avascular areas.

Anterior Chamber

The fluid-filled space between the cornea and the iris in the eye, playing a key role in maintaining intraocular pressure.

Corneal Light Reflex

The reflection of light off the cornea of the eye, used to assess the balance and alignment of the eyes.

Asymmetric Light Reflex

A condition where the reflection of light from the eyes' pupils is not equal, often indicating a potential neurological or ophthalmic issue.