Some Restriction Enzymes Do Not Leave Sticky Ends When Cutting

question 47

Multiple Choice

Some restriction enzymes do not leave sticky ends when cutting DNA molecules; rather, they cut a restriction site down the middle and leave "blunt-ended" DNA molecules, which do not have any single-stranded nucleotide extensions. Which of the following is not a likely outcome when trying to insert a gene into a plasmid when have both been cut with the same "blunt-ended" restriction enzyme?

• A)The gene might be inserted into the plasmid by forming hydrogen bonds.
• B)The gene might be inserted into the plasmid multiple times in a row.
• C)The gene might insert into the plasmid in the proper (forward)orientation.
• D)The gene might insert into the plasmid in the wrong (backward)orientation.

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