When Cloning a Gene, One of the Steps Is to Use

question 34

Multiple Choice

When cloning a gene, one of the steps is to use restriction enzymes to insert the gene of interest into a vector. If you separated the empty vector (the vector without the gene of interest) and the cloned vector (the vector that has the gene of interest added) using agarose gel electrophoresis, what do you expect to observe on the agarose gel if both vectors are loaded at the same position at the top of the gel?

• A)The empty vector would migrate farther down than the cloned vector.
• B)The cloned vector would migrate farther down than the cloned vector.
• C)The empty vector would migrate the same distance as the cloned vector.
• D)It is impossible to know without knowing the identity of the gene of interest.

---

Definitions:

Unlawful Conduct

Actions or behavior that are illegal or not permitted by law.

Economic Relations

The interactions and interdependencies between economic agents, including countries, organizations, and individuals, as they engage in production, exchange, and consumption of goods and services.

Conspiracy to Injure

A Conspiracy to Injure is an agreement between two or more parties to cause harm or detriment to another person or entity, through unlawful means or for an illegal purpose.

Intentional Infliction

Deliberate actions performed with the purpose of causing harm or distress to another party.