You Prepare a Genomic Library and Use This as a Source

question 7

Multiple Choice

You prepare a genomic library and use this as a source to produce recombinant proteins in E. coli. However, after producing the proteins you find that they are all too long or too short. In fact, you don't find a single protein that is the correct size. What is the best explanation for this result?

• A)Since a genomic library was used as the source of the genes, the introns were included during transcription and translation.
• B)Since bacteria were used to produce the proteins, sugar groups were not properly added on to the proteins that were being made.
• C)Since a genomic library was used as the source, the recombinant bacterial plasmid DNA was likely incorporated into the proteins being made.
• D)Since bacteria were used to produce the proteins, the native bacterial plasmid DNA was likely incorporated into the proteins being made.

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Definitions:

Damaged Goods

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Act of God

A legal term for natural events outside of human control, such as floods or earthquakes, which cannot be predicted or prevented.

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The intentional and malicious destruction, damage, or defacement of property without the consent of the owner.

Community Property

A legal framework in some states dictating that property acquired during a marriage is owned jointly by both spouses.