Nested Polymerase Chain Reaction (PCR) Is Very Sensitive and Specific

question 41

Multiple Choice

Nested polymerase chain reaction (PCR) is very sensitive and specific because:

• A)it uses more than one DNA polymerase to make three to four times the amount of PCR products than standard PCR.
• B)the assay itself basically serves as a form of internal control and ensures specificity.
• C)the assay will act as a form of external control, ensuring specificity and sensitivity.
• D)the fluorochrome that is attached to the primers ensures specificity of the nucleotides that are attached to the template.

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Definitions:

Morally Right

Acting in accordance with ethical principles and recognized standards of good behavior.

Morally Wrong

Actions or decisions that are considered unethical or evil based on moral principles or societal norms.

Ethical Imperatives

Moral obligations or commands that are viewed as universally true and guiding principles of behavior.

Comparative Reasoning

The process of using what is more familiar to make interpretations, explanations, or inferences about what is less familiar.