The Chemical Reaction for the Ortho-Nitrophenyl-B-D-Galactopyranoside (ONPG)test Is

question 19

Multiple Choice

The chemical reaction for the Ortho-Nitrophenyl-b-D-galactopyranoside (ONPG) test is:

• A) b-galactosidase hydrolyzes lactose into glucose and galactose
• B) Glucose ® pyruvic acid ® acetoin
• C) b-Galactosidase hydrolyzes o-nitrophenyl-b-D-galactopyranoside into galactose and o-nitrophenol (a yellow compound)
• D) O-nitrophenyl-b-D-galactopyranoside is oxidized into acetoin and glucose

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