During the Denaturation Step in Polymerase Chain Reaction (PCR)

question 25

Multiple Choice

During the denaturation step in polymerase chain reaction (PCR) :

• A) DNA polymerase extends the primers.
• B) Oligonucleotide primers are hybridized to the single-stranded DNA.
• C) Native DNA is cut into small pieces with a restriction enzyme.
• D) The target double-stranded DNA is separated into single strands during the denaturation step.

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Definitions:

Cleavage

In animal development, stage during which repeated mitotic divisions produce a blastula.

Blastula

An early stage in embryonic development that consists of a spherical layer of cells (blastomeres) surrounding a fluid-filled cavity.

Oviduct

Duct between an ovary and the uterus.

Fertilization

Fusion of two gametes to form a zygote; part of sexual reproduction.