What Is the Principle of the 5' Nuclease Assay (Taqman)

question 17

Multiple Choice

What is the principle of the 5' nuclease assay (Taqman) ?

• A) Fluorescent probe is mixed with labeled primers and a sandwich occurs where the fluorescent molecule is activated to fluorescence.
• B) The Taq DNA polymerase extends from the primers and replicates the template to which the Taqman probe is annealed. The reporter dye is released by the 5' nuclease activity of the polymerase first, then the probe is released, thus increasing the fluorescence.
• C) The Taq DNA polymerase extends from the primers and replicates the template to which the Taqman probe is annealed. Then the reporter dye is activated and the fluorescence increases with the number of hybrids that are produced.
• D) A bioluminescent probe is attached to the 5' side of the primer. As the Taq DNA polymerase begins adding nucleotides to the growing chain, the last nucleotide added (because of steric hindrance) will be the bioluminescent probe. Once added, the probe will begin luminescing and the concentration of the hybrids is directly proportional to the amount of luminescence that is produced.

Recognize the significance of European Enlightenment ideals on the American colonies.

Differentiate between various colonial revolts and their causes, especially focusing on Indian revolts against European powers.

Analyze the effects of significant wars, like the Seven Years' War, on colonial and global politics.

Understand the dynamics of slavery, the slave experience, and resistance movements within the British American colonies.

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Definitions:

Object Permanence

The concept that entities remain present even when they are not observable, audible, or detectable by any sensory means.

Paired-Associates Learning

A learning process where items to be learned are presented in pairs so that when one item of a pair is presented, the other is recalled.

Object Permanence

The understanding that objects continue to exist even when they cannot be seen, heard, or otherwise sensed, a concept crucial in cognitive development in infancy.

Conservation

The understanding that some properties of an object or substance remain the same even when its appearance is altered in some way, a concept important in cognitive development.