You Are Trying to Design a Protein That Will Be

question 18

Essay

You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E. coli is a gram-negative cell and the protein folds after it has exited the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E. coli, the protein, and the secretion system.

---

Definitions:

Standard Deviation

A measure of the amount of variation or dispersion of a set of values, indicating how much the values typically differ from the mean.

Central Tendency

Describes the center or typical value of a data set, commonly measured by the mean, median, and mode.

Range

In a general context, it refers to the extent or scope of something, such as variation between upper and lower limits on a particular scale.

Statistically Significant

A determination that a relationship between two or more variables is caused by something other than chance, based on statistical analysis.