Enumerating the Viable and Total Cell Concentration of a Population

question 19

Essay

Enumerating the viable and total cell concentration of a population of a microbial isolate can be a really laborious task for a microbiologist.Especially when a microbiologist studies the same isolate for several years,it often becomes practical to determine the relationship between optical density (OD)and cell concentration.Once this relationship (determined by a standard curve)is determined,the OD of an isolate in a broth can be routinely used to determine the population's concentration.Why must a standard curve be prepared for each isolate when using OD measurements to determine cell concentration? Also describe an experiment that would generate this type of standard curve.

Comprehend the causes and management strategies for childhood asthma.

Appreciate the influence of gender on the selection and engagement in physical activities.

Understand ADHD's impact on children's behavior and academic performance.

Comprehend the benefits of physical exercise on children's health and well-being.

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Definitions:

Air Horn

A pneumatic device designed to create an extremely loud noise for the purposes of signaling or attracting attention, commonly used in sports events and emergencies.

Neutral Stimulus

A stimulus that initially produces no specific response other than focusing attention, used in classical conditioning experiments.

Unconditioned Stimulus

In classical conditioning, a stimulus that naturally triggers a reflexive or involuntary response without the need for prior learning.

Classical Conditioning

The educational practice of regularly associating two stimuli together; a response that starts out being elicited by the second stimulus is in time elicited by only the first stimulus.