Some Proteins Overexpressed at High Levels Resulting in the Formation

question 9

Multiple Choice

Some proteins overexpressed at high levels resulting in the formation of inclusion bodies can abolish the goal of producing large quantities of active protein.What could be done to minimize this issue?

• A) Codon optimize the gene.
• B) Decrease the number of biobricks in the vector.
• C) Simultaneously produce intracellular chaperonins.
• D) Switch to an expression host with a larger intracellular volume.

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Definitions:

Antioxidants

Substances that can prevent or slow damage to cells caused by free radicals, often found in foods.

Free Radicals

Unstable atoms or molecules with unpaired electrons that can cause damage to cells, proteins, and DNA by stealing electrons from other molecules.

Longevity

The length of time that an individual lives or a thing lasts.

Maximum Life Span

The longest period an individual member of a species is known to have lived, considering genetic and environmental factors.