You Have Performed the Following Mating Experiment Using Hfr and F-Strains

question 78

Multiple Choice

You have performed the following mating experiment using Hfr and F-strains of Escherichia coli: Hfr (thr⁺ leu⁺ gal⁺ strˢ) × F⁻ (thr⁻ leu⁻ gal⁻ strʳ) . Which of the following selective media would you use to score recombinant colonies?

• A) minimal medium
• B) minimal medium + streptomycin
• C) minimal medium + threonine
• D) minimal medium + streptomycin + threonine

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