Refer to This Text to Answer the Following Questions

question 14

Multiple Choice

Refer to this text to answer the following questions.
Bacterial gene transfer is also used in the lab to introduce genes into organisms of different species,genera,phyla,or even kingdoms or domains.A major example is the use of a Ti plasmid of the soil bacterium Agrobacterium tumefaciens to transfer genes into dicotyledonous plants.The bacterium infects the plant root in the soil and transfers some of the Ti plasmid genes (T DNA) into the plant nucleus.Recombination may occur,and T DNA expression is controlled by plant compounds generated in response to wounding and infection.
-Assuming you have appropriately engineered the Ti plasmid to carry the gene in question,how could you best and most safely test whether your potatoes are now working as a vaccine?

• A)Feed the engineered potato to a population at high risk for cholera.
• B)Cook the potatoes to denature the toxin and feed them to mice susceptible to cholera.
• C)Feed pieces of raw potato to susceptible mice and test them for antibodies.
• D)Emulsify the potatoes in a liquid that can be injected into experimental animals.

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Definitions:

Ghrelin Secretions

The release of the "hunger hormone" ghrelin by the stomach, which signals the brain to stimulate appetite and intake of food.

Neophobia

The fear of anything new or unfamiliar.

Basal Metabolic Rate

How quickly the body burns energy to support fundamental processes like breathing and temperature maintenance while in a state of rest.

Energy Expenditure

The amount of energy a person uses to conduct daily activities and bodily functions.