How Might You Choose a Region of DNA for a PCR

question 26

Short Answer

How might you choose a region of DNA for a PCR primer so as to increase the temperature necessary for primer annealing (to minimize nonspecific PCR products)?

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Definitions:

Depth

The complexity or thoroughness involved in the examination or analysis of a subject, object, or concept.

Generalizability

The extent to which findings from a study can be applied or extended to broader contexts or populations outside of the initial study setting.

Relevance

The degree to which something is related or useful to the matter at hand.

Scope

The extent or range of view, reach, application, effectiveness, or influence of an object, discussion, or analysis.