The Same DNA Probe Used to Measure MRNA Levels by Northern

question 21

Multiple Choice

The same DNA probe used to measure mRNA levels by northern blotting can also be used on a Southern blot with a restriction digest of genomic DNA.What new information can be gained from Southern blotting (that wasn't already obtained by the northern blot analysis) ?

• A)the level of protein that is being translated from the mRNA transcript
• B)the presence of a unique restriction fragment pattern indicating a mutation in a known restriction site
• C)the size of the genomic DNA fragments,including both introns and exons
• D)the size of the genomic DNA fragments and the level of protein being translated
• E)the size of the genomic DNA fragments and the presence of a unique restriction fragment pattern indicating a mutation in a known restriction site

---

Definitions:

Empirical Evidence

Information acquired by observation or experimentation that is used to validate theories or hypotheses.

Crisis Situation

A critical, unstable period or state of affairs in which a decisive change is impending, often with the possibility of a highly undesirable outcome.

Minors

Individuals under the age of legal adulthood, typically unable to make certain legal decisions for themselves and subject to parental or guardian control.

Cognitive Restructuring

Cognitive restructuring is a psychological technique aimed at identifying and changing irrational or maladaptive thoughts and beliefs, often used in cognitive-behavioral therapy.