We Can Detect the SNP in the βˢ Allele Because

question 6

Essay

We can detect the SNP in the βˢ allele because it destroys a DdeI restriction site,leading to DNA restriction fragment variation revealed by Southern blot analysis.
Either two or three restriction sequences for the restriction endonuclease DdeI can occur near the β-globin gene,depending on the allele.One DdeI restriction sequence is eliminated from the βˢ allele by the SNP in the mutant allele because of a mutation in the double-stranded target sequence 5′ - CTNAG - 3′.
Suppose the wild-type allele sequence is CCTGAG,while a newly identified SNP is CCTTAG.Would this same restriction fragment analysis technique be appropriate for detecting this particular SNP? What other technique(s)could you use to identify this particular mutation?

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Definitions:

Py/o

Py/o is a prefix in medical terminology meaning pus.

Thorac/o

A prefix used in medical terms related to the chest or thoracic part of the body.

Trache/o

A combining form used in medical terms that relates to the trachea, the large airway leading from the larynx to the lungs.

Bronch/o

A combining form denoting relation to the bronchi, the major air passages of the lungs.