The Same DNA Probe Used to Measure MRNA Levels by Northern

question 21

Multiple Choice

The same DNA probe used to measure mRNA levels by northern blotting can also be used on a Southern blot with a restriction digest of genomic DNA.What new information can be gained from Southern blotting (that wasn't already obtained by the northern blot analysis) ?

• A)the level of protein that is being translated from the mRNA transcript
• B)the presence of a unique restriction fragment pattern indicating a mutation in a known restriction site
• C)the size of the genomic DNA fragments,including both introns and exons
• D)the size of the genomic DNA fragments and the level of protein being translated
• E)the size of the genomic DNA fragments and the presence of a unique restriction fragment pattern indicating a mutation in a known restriction site

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Definitions:

Propanol

An alcohol with the chemical formula C3H8O, used as a solvent and antiseptic.

Balanced Equation

A chemical equation where the number of atoms for each element is equal on both the reactant and product sides, indicating conservation of mass.

Methane Gas

A colorless, odorless flammable gas that is the simplest alkane, consisting of one carbon atom and four hydrogen atoms, widely used as a fuel.

Complete Combustion

A chemical reaction in which a compound reacts with an oxygen-rich environment to produce a complete oxidation, releasing maximum energy.