We Can Detect the SNP in the βˢ Allele Because

question 6

Essay

We can detect the SNP in the βˢ allele because it destroys a DdeI restriction site,leading to DNA restriction fragment variation revealed by Southern blot analysis.
Either two or three restriction sequences for the restriction endonuclease DdeI can occur near the β-globin gene,depending on the allele.One DdeI restriction sequence is eliminated from the βˢ allele by the SNP in the mutant allele because of a mutation in the double-stranded target sequence 5′ - CTNAG - 3′.
Suppose the wild-type allele sequence is CCTGAG,while a newly identified SNP is CCTTAG.Would this same restriction fragment analysis technique be appropriate for detecting this particular SNP? What other technique(s)could you use to identify this particular mutation?

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Definitions:

Bones

Rigid organs that constitute part of the endoskeleton of vertebrates, supporting body structures and facilitating movement.

Large Arteries

Blood vessels that carry oxygen-rich blood away from the heart to various parts of the body, characterized by their large diameter and elastic walls.

Connective Tissue

A type of biological tissue that supports, protects, and binds other tissues in the body.

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The resources, knowledge, and skills passed down within families that can influence an individual's life chances and educational outcomes.