You Are Trying to Prepare a DNA Karyotype,but This Time

question 5

Multiple Choice

You are trying to prepare a DNA karyotype,but this time you forgot to add trypsin to your chromosome preparation.What effect would you expect to see?

• A)Dyes would stain the chromosomes darker because more proteins are present to take up the dye.
• B)Dyes would not stain the chromosomes as expected,because proteins would not be removed from the chromatin and so would block the dye from accessing the DNA.
• C)Chromosomes do not condense properly,so it would be very difficult to isolate individual chromosomes.
• D)Chromosomes would be degraded quickly because the trypsin is necessary to promote DNA stability by adding more scaffold proteins to the chromatin.
• E)The chromosomes would be more difficult to pair up because the trypsin acts as a dye,creating a banding pattern that distinguishes between chromosomes.