Some Restriction Enzymes Cut at Specific and Unambiguous Sequences (E

question 25

Multiple Choice

Some restriction enzymes cut at specific and unambiguous sequences (e.g.,NotI at GCGGCCGC) .As such,the distance between two such sites in a large random DNA sequence is 4⁸.However,the sequence recognized by XhoII is said to be ambiguous (Pu-GATC-Py,where Pu = any purine and Py any pyrimidine) .What,therefore,is the expected distance between two XhoII sites in a large random sequence?

• A)4 × 4 × 4 × 4
• B)1 × 4 × 4 × 4 × 4 × 1
• C)2 × 4 × 4 × 4 × 4 × 2
• D)4⁶
• E)4² × 2²

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Definitions:

Emerson

Ralph Waldo Emerson, an American essayist, lecturer, philosopher, and poet who led the transcendentalist movement of the mid-19th century.

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Refers to Nathaniel Hawthorne, an American novelist and short story writer known for his novels "The Scarlet Letter" and "The House of the Seven Gables."

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Herman Melville was an American novelist, short story writer, and poet best known for his novel "Moby Dick," which explores complex themes including humanity's struggle against nature.