In Producing a Recombinant Plasmid to Be Used to Clone

question 14

Multiple Choice

In producing a recombinant plasmid to be used to clone a given donor insert, it is possible to cut both the donor and plasmid with the same restriction enzyme, resulting in complementary sticky ends. Assuming plenty of plasmid DNA is available, why is further selection necessary before the introduction of the plasmid into a cellular system?

• A)Contamination will have introduced other donor inserts.
• B)Some donor inserts will be single stranded and deteriorate.
• C)Some donor inserts will be sensitive to particular antibiotics.
• D)Some donor pieces will remain uninserted.
• E)Some donor strands will be inserted with an incorrect orientation.

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Definitions:

Self-control

The ability to regulate one's emotions, thoughts, and behaviors in face of temptations and impulses.

Autism Spectrum Disorder

A developmental disorder characterized by challenges with social skills, repetitive behaviors, and speech and nonverbal communication, with symptoms appearing in early childhood.

Intellectual Development Disorder

A disorder characterized by significant limitations in both intellectual functioning and adaptive behavior, impacting everyday social and practical skills.