The Following Table Presents the Effect of Different Media on the Growth

question 13

Essay

The following table presents the effect of different media on the growth response of tryptophan mutations in Salmonella typhimurium (+ = growth,- = no growth).
$\begin{array}{r}\underline {\text { Medium Supplemented with } } \\\begin{array}{cccc} \text { Strain } & \text { No Supplement } &\text { IGP } & \text { AA } & \text { IN } & \text { TRY } \\\hline\operatorname{trp}-8 &-&+ & + & + & + \\\operatorname{trp}-2 &-&+ & - & + & + \\\operatorname{trp}-3 &-&- & - & + & + \\\operatorname{trp}-1&-&- & - & - & +\end{array}\end{array}$ (a) Construct the biochemical pathway for the compounds IGP,AA,IN,and TRY based on these data.
(b) Place strains of bacteria (mutations)in the appropriate steps in the pathway.
(c) In bacteria it is often possible to make partial diploid strains.Assume that a diploid strain was made containing the complete genomes of the trp-2 and trp-1 strains.Would this diploid strain be able to grow on the unsupplemented medium? Explain your answer.

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Definitions:

Molecule

The smallest unit of a chemical compound that can exist independently while retaining the composition and chemical properties of the compound.

Cis-trans Isomers

Isomers that differ in their spatial arrangement of groups around a double bond or within a ring, affecting the physical and chemical properties of the molecules.

Constitutional Isomers

Molecules that have the same molecular formula but different connections among their atoms.

Isomeric

Relates to molecules with the same molecular formula but different arrangements of atoms in space.