As Part of an Experiment Where Absorbance Values Are Measured

question 34

Essay

As part of an experiment where absorbance values are measured using a spectrophotometer,you are taking readings of your sample every 20 minutes.The non-motile microbe you're testing has a generation time of roughly 20 minutes at an incubation temperature right around room temperature.Things start out fine,with the expected results - as time goes by at the correct incubation temperature,absorbance starts to rise as the medium starts to become more cloudy with growing microorganisms.But roughly 2 hours into the process,you notice that the absorbance levels flatten out,and then start to decrease unexpectedly.What is most likely taking place in your experiment?

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