Place the Steps of Constructing a Genomic Library in Order

question 19

Multiple Choice

Place the steps of constructing a genomic library in order.
I.Digest phage with restriction enzyme.
II.Lyse cells of interest and precipitate DNA.
III.Introduce constructs via transformation into competent E.coli cells.
IV.Perform ligation reaction with genome fragments and phage.
V.Digest genomic DNA with restriction enzyme.

• A)V,IV,I,III.II.
• B)I,II,III,IV,V.
• C)II,I,III,V,IV.
• D)II,V,I,IV,III.
• E)IV,II,III,I,V.

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