Place the Steps of Constructing a Genomic Library in Order

question 19

Multiple Choice

Place the steps of constructing a genomic library in order.
I.Digest phage with restriction enzyme.
II.Lyse cells of interest and precipitate DNA.
III.Introduce constructs via transformation into competent E.coli cells.
IV.Perform ligation reaction with genome fragments and phage.
V.Digest genomic DNA with restriction enzyme.

• A)V,IV,I,III.II.
• B)I,II,III,IV,V.
• C)II,I,III,V,IV.
• D)II,V,I,IV,III.
• E)IV,II,III,I,V.

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Definitions:

Vaginal Pain

Discomfort or pain experienced in the vaginal area, which can be caused by a variety of medical or psychological conditions.

Sexual Intercourse

The physical act of sex between individuals, typically involving penetration and aimed at sexual pleasure or reproduction.

AIDS

Acquired Immunodeficiency Syndrome, a chronic, potentially life-threatening condition caused by the human immunodeficiency virus (HIV) that damages the immune system.

Unprotected Sex

Engaging in sexual activities without the use of contraceptives or barrier methods, increasing the risk of sexually transmitted infections (STIs) and unwanted pregnancies.