You Insert a Gene for Tetracycline Resistance into One Plasmid

question 55

Multiple Choice

You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid of the same incompatability group.You successfully introduce both plasmids into a sample of E.coli cells,and surprisingly succeed in obtaining growth in culture medium with both antibiotics present in it.What likely happened?

• A)Random mutation has activated the antibiotic resistance genes
• B)Plasmid incompatibility will not allow both plasmids to persist
• C)E.coli cannot maintain two plasmids so they have integrated
• D)One or more plasmid(s)have integrated into the bacterial chromosome
• E)A phage has neutralized one of the plasmids

---

Definitions:

Allowance

A reduction from the gross amount of an asset or liability, created to account for potential future adjustments such as doubtful debts or returns.

Processing Fee

A charge assessed for processing a transaction, application, or request, common in financial services.

Bank Credit Cards

Financial instruments issued by banks that allow users to borrow funds for purchases or cash advances, subject to repayment terms and interest.

Journal Entry

A record of a financial transaction in an accounting system, showing the accounts affected and the amounts.