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Which Restriction Endonuclease Do You Predict to Generate DNA Fragments

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Which restriction endonuclease do you predict to generate DNA fragments which are most difficult to join with DNA ligase?


Definitions:

Alertness

The state of being watchful and prompt to meet danger or emergency, or to discover and avoid errors.

Opener

An initial statement or action that serves to open a conversation, meeting, or written document.

Presentation

A formal display or demonstration of information or products, often before an audience, using various media such as slides or videos.

Single Purpose

A focused objective for a piece of writing, ensuring that all content contributes to this main goal.

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