After PCR,the Amplification Control Has Failed to Yield a Product

question 3

Multiple Choice

After PCR,the amplification control has failed to yield a product.What should you do?

• A)Report out Indeterminate
• B)Report out Negative because there was no amplification from the sample
• C)Check the original DNA or RNA preparation.If it is adequate,repeat the amplification.If not,re-isolate the nucleic acid.
• D)Repeat it using a higher concentration of amplification control in the reaction.

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Definitions:

Characters

The symbols, including letters, numbers, and punctuation marks, that can be typed or displayed on a screen.

256

Typically refers to a 256-bit encryption level or a color depth of 256 colors in computing contexts.

128

Typically refers to the binary representation of data or can indicate a specific value or quantity in various contexts.

64

Typically used in the context of computing to refer to 64-bit architecture, which allows for more efficient processing and access to greater amounts of memory compared to 32-bit.