While Developing a New Hybridization-Based Molecular Test for Cryptosporidium,a Protozoan

question 2

Multiple Choice

While developing a new hybridization-based molecular test for Cryptosporidium,a protozoan parasite that causes diarrheal illness,a laboratory technician examines the hybridization ability with a closely related,but nontarget,protozoan and also gets a positive test result.In order to improve the stringency of the hybridization reaction for detection of Cryptosporidium only,the technician can do which of the following?

• A)Decrease the salt concentration in the hybridization buffer
• B)Increase the salt concentration in the hybridization buffer
• C)Reduce the reaction temperature during hybridization
• D)Remove destabilizing agents,such as formamide,from the hybridization buffer
• E)None of the above

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Definitions:

Administering Medications

The process of giving drugs to a patient as prescribed, which can be oral, intravenous, or through other routes.

Sterile Technique

A set of specific practices and procedures in medical settings aimed at eliminating all microorganisms from an object or area to prevent infection.

Tracheostomy Care

The maintenance and care procedures for a tracheostomy, which includes cleaning and changing the tracheostomy tube and monitoring for complications.

Acute Care Setting

A branch of secondary health care where a patient receives active but short-term treatment for a severe injury or episode of illness, an urgent medical condition, or during recovery from surgery.