Place the Basic Steps Involved in a Molecular Hybridization Assay

question 14

Multiple Choice

Place the basic steps involved in a molecular hybridization assay in sequence from first to last in the order in which they would be performed.

• A)Heating sample to obtain single-stranded target nucleic acid,hybridization of target to probe,detection of hybridization,labeling of probe
• B)Labeling of probe,heating sample to obtain single-stranded target nucleic acid,hybridization of target to probe,detection of hybridization
• C)Hybridization of target to probe,detection of hybridization,labeling of probe,heating sample to obtain single-stranded target nucleic acid
• D)Hybridization of target to probe,labeling of probe,heating sample to obtain single-stranded target nucleic acid,detection of hybridization

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Definitions:

Phenotypes

The observable characteristics or traits of an organism that result from the interaction of its genotype with the environment.

Recessive Traits

Traits that are expressed in an organism's phenotype only when two copies of the recessive allele are present.

Blue Eyes

An eye color resulting from a lower concentration of melanin in the iris, allowing light to scatter in a way that makes the eyes appear blue.

Cystic Fibrosis

Cystic Fibrosis is a genetic disorder that affects the respiratory and digestive systems, leading to thick mucus production and respiratory infections.