Quality Control Results for Disk Diffusion AST Using Appropriate ATCC

question 29

Multiple Choice

Quality control results for disk diffusion AST using appropriate ATCC strains of bacteria showed zone size diameters decreasing over time for a few antimicrobials.Which of the following statements is a possible explanation?

• A)Antimicrobial disks were stored in a frost-free freezer.
• B)Concentration of the bacterial inoculum was 5× 10⁵ CFU/mL.
• C)Depth of Mueller-Hinton agar plates measured 3 mm.
• D)Plates were incubated less than 16 hours in ambient air.

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