Why Are Dideoxynucleotides Added to the Reaction in the Sanger

question 2

Multiple Choice

Why are dideoxynucleotides added to the reaction in the Sanger sequencing methodology?

• A) They label the DNA strands
• B) They cause termination of the replicating strand
• C) They allow longer processivity of the DNA polymerase
• D) They cement the newly synthesized strand to the template strand
• E) There is a lower chance of mutation during the sequencing reaction

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