A His-Tag or Strep-Tag Is Part of the Cloning Area

question 37

Multiple Choice

A his-tag or strep-tag is part of the cloning area in expression vectors.How do these sequences function?

• A) To increase gene expression from the cloned DNA.
• B) In regulation of expression of the cloned DNA.
• C) As unique restriction enzyme sites to aid in cloning DNA.
• D) Affinity sequences to aid in purification of the recombinant protein.
• E) As signal sequences for the secretion of the recombinant protein.

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Definitions:

Exudative

Pertaining to the oozing or discharge of fluid from a wound or body cavity.

Hydrocolloid Dressing

A type of adhesive dressing that forms a gel when in contact with wound fluid, providing moist wound healing and protection against infection.

Second Intention

A method of wound healing where the wound is left open and allowed to close naturally without surgical closure.

Heavy Exudate

A condition characterized by the release of large amounts of fluid and proteins from blood vessels into surrounding tissues, commonly seen in wounds.