A His-Tag or Strep-Tag Is Part of the Cloning Area

question 37

Multiple Choice

A his-tag or strep-tag is part of the cloning area in expression vectors.How do these sequences function?

• A) To increase gene expression from the cloned DNA.
• B) In regulation of expression of the cloned DNA.
• C) As unique restriction enzyme sites to aid in cloning DNA.
• D) Affinity sequences to aid in purification of the recombinant protein.
• E) As signal sequences for the secretion of the recombinant protein.

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