A Pharyngeal Specimen Is Inoculated on Cystine-Tellurite Blood Agar,modified Tinsdale

question 3

Multiple Choice

A pharyngeal specimen is inoculated on cystine-tellurite blood agar,modified Tinsdale agar,and MacConkey agar.After 48 hours of incubation,black colonies with a brown halo are observed on the Tinsdale agar and gray colonies are isolated from the cystine-tellurite agar.However,no growth is observed on the MacConkey agar.Which one of the following statements explains this discrepancy?

• A)The MacConkey agar is most likely expired.
• B)The MacConkey agar should be incubated an additional 24 hours to observe growth.
• C)The pH of the media may be altered,causing an environment that is too acidic for the organism to grow.
• D)No discrepancy is present;this organism does not grow on MacConkey agar.

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